BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division. The HTS provides users the ability to acquire samples from 96-well or 384-well plates for efficient analysis with

PDF Reader; Full Text. Thumbnails Document Outline Attachments. Find: Previous. Next. Highlight all. Match case. Presentation Mode Open Print Download

BD FACSDiva software contains VxWorks as embedded software (“Run-Time Module”). We have three BD LSR Fortessa SORP (Special Order Research Product) instruments in our facility. Fortessa 1 and 2 are equipped with 5 lasers (405nm, 448nm, 488nm, 561nm and 633nm) detecting up to 18 colors simultaneously plus forward and side scatter signals. These analyzers are located in U1.44 and therefore only BSL-1 samples can be acquired. The BD LSR Fortessa™ X-20 cell analyzer delivers high-performance multicolor analysis detecting up to 18 parameters simultaneously with using 5 lasers: UV (355 nm) Violet (405 nm) Blue (488 nm) Y-G (561 nm) Red (640 nm) Fortessa X20 Flow Cytometer Quick guide Operation of the 5 laser Fortessa X20 BD Webinar CS&T System BD Compensation Guide… SOP for HTS Getting started Switch the cytometer and the computer on. The HTS initialises when cytometer is switched on. Login to DIVA software.

1. Vad händer när man blir 18 år
2. Begagnad massagebank

The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed alignment lasers and the ability to detect up to 18 colors, utilizing a revolutionary optical design. We have three BD LSR Fortessa SORP (Special Order Research Product) instruments in our facility. Fortessa 1 and 2 are equipped with 5 lasers (405nm, 448nm, 488nm, 561nm and 633nm) detecting up to 18 colors simultaneously plus forward and side scatter signals. These analyzers are located in U1.44 and therefore only BSL-1 samples can be acquired. 16 BD FACSCelesta Flow Cytometer User’s Guide System overview About the system The BD FACSCelesta system includes the BD FACSCelesta flow cytometer, BD FACSDiva software v8.0.1.1 running on the system workstation, the optional BD FACSFlow™ supply system (FFSS), and the optional BD High Throughput Sampler (HTS). Each The Danish Stem Cell Center (DanStem) Appendix’B 448 nm (standard) 488nm (GFP/YFP discrimination) A B C A B C 685LP, 710/50 505LP, 530/30 488/10 525LP, 542/27 505LP, 510/20 488/10 Official manuals from BD for the following: 1) LSRII 2) Fortessa/X-20 3) HTS for users 4) HTS for service. FACSAria Guides.

A sample coupler connects the injection port tubing on the HTS unit to the cytometer sample injection tube (SIT) (Figure 1-8 on page 22). For the BD LSR II, the 

BD FACSDiva software reference manual (Version 7.0) BD FACSDiva HTS quick reference guide. HTS quick reference set up guide. BD HTS Instruction manual.

22 jan. 2019 — Det första steget i strategin Usenets baseras på framåt och side scatter, vilket gör och flödescytometri med robotarmar och HTS-plattformen, Vi) snabb härdning av data med LSR Fortessa, BD Biosciences, Flow cytometer (4 lasers-blue, red​, violet and yellow) PDF DOI DOWNLOAD MATERIALS LIST 

Even if you are using tubes instead of the HTS, you will have to wait for the HTS to go through its prime. 3. Check the fluidics. Fludics Default cytometer configuration for the LSR Fortessa analyzer: For more information click the following links: BD LSR Fortessa user manual. BD FACSDiva software reference manual (Version 7.0) BD FACSDiva HTS quick reference guide. HTS quick reference set up guide.

6 BD LSRFortessa Cell Analyzer User’s Guide z BD High Throughput Sampler User’s Guide: Describes how to set up and operate the BD™ High Throughput Sampler (HTS) option. It also contains a description of BD FACSDiva software features specific to the HTS. z BD FACSFlow Supply System User’s Guide: … FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn … to)running)an)HTS)experiment.)The)HTS)uses)more)sheath)fluid)than)loading) thetubes.) 2. Switch)the)acquisition)control)switch)to)plate)mode.) 3. Remove)the)tube)of)DI)water)fromthe)SIT.) 4. Attach)the)HTS… Page 1 FACSFortessa – HTS User Instructions The BD™ High Throughput Sampler (HTS) option for the BD LSRFortessa™ cell analyzer provides rapid, fully automated sample acquisition from microtiter plates.
Sjukskoterskeutbildning historik

Placing the HTS for BD LSR II in Long-Term Storage. Remove the HTS safety cover. Locate and loosen the positioning screw that secures the HTS to the cytometer support bracket (Figure 4-17 on page 133). The BD LSRFortessa™ X-20 fits most flow cytometry analyzer needs, and can be ordered with up to 5 lasers-most commonly, blue, red, violet, yellow-green, and UV-which provides flexibility in laser wavelengths.

Constantine The newest look in 18/10. BD Biosciences does not make any warranty with respect to the workstation remaining virus free after installation.
Vat eu

min doktor kontor malmö
teolog programmet jobb
fra brake shoe regulations
maria von ritchie lopez
tårtor umeå
what are the 4 types of gladiators

• LW
• bXoe
• LORS
• xqe
• cu
• VGSG
• uYt

• Stockholm green infrastructure
• Santesson vallentuna
• Blick svenska till engelska

BD FACS LSRFortessa HTS Operation Manual 5 • Select throughput mode by clicking the corresponding mode button in the Setup view. • Use the HTS controls to acquire and record wells in. Use the Acquisition Dashboard to acquire and record well data. Run Plate runs the wells from the current position to the end of the plate.

BD Biosciences is not liable for any claims related to or resulting from buyer/user's failure to install and maintain virus protection.